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详细介绍
Medium 199 培地是根据 Morgan、Morton 和 Parker 的合成培养基配方，经过岛津特殊的制备方法调制而成的粉末培养基。
该培养基包含各种氨基酸、维生素、核酸成分和少量的中间代谢物等。单独使用该培养基不能期望细胞增殖，但在脊髓灰质炎疫苗和其他病毒研究中被广泛用作维持细胞的培养液。此外，添加血清后，该培养基在初代培养的细胞增殖方面非常出色。
称取 9.5g 该粉末培养基溶解于蒸馏水中，制成 1000mL 溶液（禁止加热）。加入适量（加入 1.3-2.2g 后，在 5% CO₂ 下，37℃时 pH 值为 7.1-7.4），溶解后立即进行过滤灭菌。预先使用 CO₂ 气体调节 pH 值后再进行过滤可以防止过度的 pH 升高。如不立即使用，应密封保存在阴凉处（2-10℃）。在使用前，根据需要加入适量的血清。
该粉末培养基需要密封保存，并放置在干燥阴凉避光处（2-10℃）。
生产之后一年。
每 9.5g (1 L)
Sodium Chloride................................ 6,800 mg
Potassium Chloride............................. 400 mg
Calcium Chloride(Anhydrate).................. 200 mg
Magnesium Sulfate(Anhydrate)................ 97.7 mg
Sodium Dihydrogen Phosphate(Monohydrate).... 125 mg
Iron(III)Nitrate(Enneahydrate)................ 0.72 mg
Sodium Acetate................................. 50 mg
Adenosine 5' Triphosphate Disodium........... 1 mg
Glucose.......................................... 1,000 mg
L-Arginine Hydrochloride...................... 70 mg
L-Histidine Hydrochloride Hydrate............ 21.9 mg
L-Lysine Hydrochloride........................ 70 mg
L-Tryptophan.................................... 10 mg
L-Phenylalanine............................... 25 mg
L-Methionine................................... 15 mg
L-Serine........................................ 25 mg
L-Threonine..................................... 30 mg
L-Leucine...................................... 60 mg
L-Isoleucine................................... 20 mg
L-Valine........................................ 25 mg
L-Gutamic Acid................................ 66.8 mg
L-Aspartic Acid............................... 30 mg
L-Alanine....................................... 25 mg
L-Proline....................................... 40 mg
L-Hydroxyproline............................. 10 mg
Glycine......................................... 50 mg
L-Glutamine................................... 100 mg
L-Cystine...................................... 20 mg
L-Tyrosine...................................... 40 mg
L-Cysteine Hydrochloride Hydrate.............. 0.11 mg
p-Aminobenzoic Acid........................... 0.05 mg
D-Biotin........................................ 0.01 mg
Calcium Pantothenate.......................... 0.1 mg
Folic Acid...................................... 0.01 mg
myo-inositol................................... 0.05 mg
Nicotinic Acid................................ 0.025 mg
Nicotinamide................................... 0.025 mg
Pyridoxal Hydrochloride...................... 0.025 mg
Pyridoxine Hydrochloride...................... 0.025 mg
Riboflavin...................................... 0.01 mg
Thiamine Hydrochloride........................ 0.01 mg
Vitamin A Acetate............................ 0.1 mg
L-Ascorbic Acid............................... 0.05 mg
α-Tocopherol Disodium Phosphate............. 0.01 mg
Calciferol..................................... 0.1 mg
2-Methyl-1,4-Naphthokine..................... 0.01 mg
Adenine......................................... 0.9 mg
2-Deoxy-D-Ribose............................. 0.5 mg
Guanine......................................... 10 mg
Guanine Hydrochloride........................ 0.3 mg
Hypoxanthin................................... 0.3 mg
Thymine........................................ 0.3 mg
Uracil........................................... 0.3 mg
Xanthine........................................ 0.3 mg
AMP -2 Na..................................... 0.2 mg
ID-Ribose...................................... 0.5 mg
Glutathione................................... 0.05 mg
Cholesterol................................... 0.2 mg
Polysorbate 80............................... 5 mg
Phenol Red...................................... 6 mg
Other substances to be added
Sodium Bicarbonate.......................... As Needed
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